고용량의 Dexamethasone 존재하에서 골막기원세포에서 발현되는 혈관신생인자의 평가
EVALUATION OF ANGIOGENIC PHENOTYPES IN CULTURED HUMAN PERIOSTEAL-DERIVED CELLS UNDER HIGH-DOSE DEXAMETHASONE
박봉욱, 최문정, 류영모, 이성균, 하영술, 김덕룡, 조영철, 김종렬, 변준호,
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박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
최문정 ( Choi Mun-Jeoung ) - 경상대학교 의과대학 구강악안면외과학교실
류영모 ( Ryu Young-Mo ) - 경상대학교 의과대학 구강악안면외과학교실
이성균 ( Lee Sung-Gyoon ) - 경상대학교 의과대학 구강악안면외과학교실
하영술 ( Hah Young-Sool ) - 경상대학교 의과대학 생화학교실
김덕룡 ( Kim Deok-Ryong ) - 경상대학교 의과대학 생화학교실
조영철 ( Cho Yeong-Cheol ) - 울산대학교 의과대학 구강악안면외과학교실
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
KMID : 0360120080300030217
Abstract
Angiogenesis plays an important role in bone development and postnatal bone fracture repair. Vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptors (VEGFRs) have been thought to be primarily involved in promoting angiogenesis. It is well known that VEGF and its receptors have been reported to play an important role in the regulation of the interaction between angiogenesis and osteogenesis during bone repair processes. Dexamethasone, a potent synthetic glucocorticoid, promotes phenotype markers of osteoblast differentiation, such as ALP and osteocalcin. It stimulates in vitro osteogenesis of human bone marrow osteogenic stromal cells. Dexamethasone has been reported to suppress VEGF gene expression in some cells. However, our previous study demonstrated VEGF quantification increased in a time-dependent manner in periosteal-derived osteogenesis under dexamethasone. So, the purpose of this study was to examine the angiogenic phenotypes in cultured human periosteal-derived cells under high-dose dexamethasone. Periosteal-derived cells were cultured using a technique previously described. After passage 3, the periosteal-derived cells were further cultured for 28 days in an osteogenic inductive culture medium containing ascorbic acid, -glycerophosphate and high-dose dexamethasone, We evaluated the expression of VEGF isoforms, VEGFR-1, VEGFR-2, and neuropilin-1, ALL VEGF isoforms (, and ) expression was observed by RT-PCR analysis. VEGFR-1, VEGFR-2 and neuropilin-1 expression increased up to day 14, particularly during the early stage of mineralization. Our results suggest the involvement of direct VEGFs/VEGFRs system on periosteal-derived cells during early mineralization phase under high-dose of dexamethasone. These also suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
키워드
Periosteal-derived cell;High-dose dexamethasone;VEGF;VEGFR;Autocrine growth molecule
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